| GALT

Galactose-1-phosphate uridyltransferase (or GALT, G1PUT) is an enzyme responsible for converting ingested galactose to glucose.Galactose-1-phosphate uridyltransferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely: UDP-glucose + galactose 1-phosphate  ⇌ {\displaystyle \rightleftharpoons } glucose 1-phosphate + UDP-galactose The expression of GALT is controlled by the actions of the FOXO3 gene. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.28	1.82	1.23	0.8	0.53	0.36	0.23	0.08
Corrected OD	2.2	1.74	1.15	0.72	0.45	0.28	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat GALT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Culture Supernates(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Rat GALT and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	85-95	88-96
	Average (%)	91	94
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98